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Sorry for self promotion but you might also consider the HILIDE method which is very similar to bicelle and sponge, only having fully solubilized protein:lipid:detergent complexes as input sample and therefore being fully compatible with regular liquid handling robotics. See Gourdon P et al 2011, Crystal Growth & Design 11, 2098-2106 for the method, and Sonntag Y et al 2011, Nature Comm 2, 304 for a direct look at the bilayers formed in the crystal Poul On 16/02/2012, at 23.53, Cory Brooks <[log in to unmask]> wrote: > Hello all; > > I am wondering if anyone has any experience getting a ARI Gryphon robot to > pick up protein in a bicelle solution? > The nano needle on our robot does not seem to want to suck the sample up > in a variety of liquid class settings. > Any suggestions out there on if this can be made to work? > > Cheers, > Cory > > > Cory Brooks,Ph.D. > > University of Alberta > Postdoctoral Fellow