Just a reminder. Quickchange is not PCR. It is linear amplification. It is very hard to see a band in the gel if you follow the standard protocol.<br><br>Nian<br><br><div class="gmail_quote">On Fri, Feb 3, 2012 at 12:14 PM, Fred <span dir="ltr">&lt;<a href="mailto:[log in to unmask]"><a href="/cgi-bin/wa-jisc.exe?LOGON=A3%3Dind1202%26L%3DCCP4BB%26E%3Dquoted-printable%26P%3D324185%26B%3D--20cf303dd29e906c7e04b8195b6c%26T%3Dtext%252Fhtml%3B%2520charset%3DISO-8859-1%26pending%3D" target="_parent" >[log in to unmask]</a></a>&gt;</span> wrote:<br>
<blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">Hi CCP4 list,<br>
Thanks everyone who have answered my post concerning to mutagenesis.<br>
From quick reading most of the answers, the following seems to be a consensus:<br>
1) Do not concentrate your PCR product;<br>
2) Too much DNA and/or impurities like salts or whatever can inhibits transformation;<br>
3) Purify your PCR product before transformation if possible or use 3 of 4 microL of it. This is more or less the amount of DNA showed in the uploaded image.<br>
Kind regards,<span class="HOEnZb"><font color="#888888"><br>
Fred<br>
</font></span><br>
P.S.: I&#39;ll let you know the results.<br>
</blockquote></div><br>