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If you want test a lot of different conditions a Thermofluorecence assay might
work for your protein and you may find a condition which stabilise your
protein. However there is no warranty that it crystallise better afterwards.

Christian

Am Montag 27 Februar 2012 17:01:33 schrieb Sangeetha Vedula:
> Dear bb users,
>
> I am trying to crystallize a ~320 kDa protein that crashes out if
> concentrated past about 3 mg/mL.
>
> I would like to try to exchange it into various buffer-salt-additive
> combinations to see which buffer works. For a starting point, I'd like to
> use desalting colums.
>
> Does anyone have suggestions for good buffer exchange and sample recovery?
> I woud like to load about 250 uL onto each column.
>
> Thanks a lot!
>
> Best regards,
>
> Sangeetha.
>