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Hi i do not want to get into trouble by going against any products The plates what you are talking about as soon it came out we tested. I did not look back to see if there was a monoolein:cholestrol coated plate. The ones i used were for sure monoolein coated ones. That tells it all.The rest of the plates still unused. I set up plates with bacteriorhodopsin even that did not work in our hands (the bacteriorhodopsin set up otherwise would work very well.) By the way it did not work for me did not mean it is bad. And the plates were in plasticware made it highly birifringent to document the results. But the plates Patrick says here i am not sure about. If Patrick want to to tell me a little bit more about i would like to hear that. I would set up plates the normal way. I am really not convinced mesophase will form by just adding protein-detergent to a previously poured monoolein. anyway i am also interested to hear experts views of the method adapted in this product. There is a poster that they shown at their website shows bacteriorhodopsin and another membrane protein crystallized using these plates. These are my personal opinion. Pius On Sat, Feb 25, 2012 at 3:35 PM, Yuri Pompeu <[log in to unmask]> wrote: > Hello Everyone, > I am considering the purchase of crystallization plates for membrane proteins. > I would love to hear what some of the community thinks or has experienced with these. > Particulalrly the monoolein and monoolein/cholesterol coated plates ( I am not sure I can mention the vendor here but it "should" not matter) > So fire away. Is it worth it? Any succes stories? Bad experiences? > I appreciate the input > Best, > Yuri -- Pius S Padayatti,PhD, Phone: 216-658-4528