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Just a reminder. Quickchange is not PCR. It is linear amplification. It is very hard to see a band in the gel if you follow the standard protocol. Nian On Fri, Feb 3, 2012 at 12:14 PM, Fred <[log in to unmask]> wrote: > Hi CCP4 list, > Thanks everyone who have answered my post concerning to mutagenesis. > From quick reading most of the answers, the following seems to be a > consensus: > 1) Do not concentrate your PCR product; > 2) Too much DNA and/or impurities like salts or whatever can inhibits > transformation; > 3) Purify your PCR product before transformation if possible or use 3 of 4 > microL of it. This is more or less the amount of DNA showed in the uploaded > image. > Kind regards, > Fred > > P.S.: I'll let you know the results. >