Dear SPM experts I´m trying to run a gPPI analysis using the Donald McLaren´s toolbox and I would like to ask you some questions. My task, it´s an even related design divided in 3 runs (213, 218 and 214 volumes respectively) with 8 conditions (conditions are the same across runs). SPM matrix for first level analysis was performed with SPM5. I introduce parameters in P structure as follow: P = Subject={' subject_1'} VOI={ 'F:\gppi \ROIS\ROI_Amygdala_LB.img'} Region={ 'ROI_Amygdala_LB'} SPMver= 5 Directory='F:\ gppi \ subjects\subject_1\ ' Estimate= 1 Contrast={'Omnibus F-test for PPI Analyses'} Extract= 'mean' Tasks={'1' 'A' 'B' 'C' 'D' 'E' 'F' 'G' 'H'} Weights= [] Maskdir= [] Equalroi= 1 FLmask=0 VOI2={} Analysis= 'psy' Method= 'cond' CompContrasts= 1 Weighted= [] Contrasts= P.Contrasts.name='A>B' P.Contrasts.STAT='T' P.Contrasts.left={'A'} P.Contrasts.right={'B'} P.Contrasts.Weighted=[] P.Contrasts.Prefix={'Sn(1)' 'Sn(2)' 'Sn(3)'} P.Contrasts.Contrail= {'bf(1)'} P.Contrasts.MinEvents=1 Matlab returns the subsequent error: 'Program will exit. Contrast cannot be defined.' 1) My first question is, is there something wrong in P structure? Since the erros seems to related to contrasts generation, I tried to avoid that toolbox generate contrasts automatically. Then, I performed with SPM an Omnibus F-test (named Omnibus) and changed in above P structure the subsequents parameters: Contrast= {'Omnibus' } CompContrasts= 0 (following the guide in this way any contrast will be generated) Then, the matrix was generated and estimated, but contrasts as expected, were not defined (Note that if I change only one of the two parameters above, toolbox doesn´t run and the same error appears; 'Program will exit. Contrast cannot be defined.'). The resulting matrix is as follow: A B C D E F G H PPI_A PPI_B PPI_C PPI_D PPI_E PPI_F PPI_G PPI_H ROI_seeddtc and 6 rp_mov (for run 1 run2 and run3) 2) My second question is if the correct contrast to obtain connectivity differences during A>B, is simply put 1 for PPI_A regressor in each run, -1 for PPI_B regressor in each run and 0 for any other columns? Thanks in advance. Regards Victor.