Hi everyone, I need a timing estimation for a study of tbss for a big amount of subject (N = 50, N=100, or even N=150), and I saw in www.fmrib.ox.ac.uk/fsl/tbss/index.html one formula that there is an approximation for the non linear registration (tbss_2_reg) for all-subjects-to-all-subjects options ( 5min x N x N). I did an experiment for N = 4, N = 8, N=13 using t test and this options for N=13: fsl4.1-tbss_1_preproc *.nii.gz fsl4.1-tbss_2_reg -n fsl4.1-tbss_3_postreg -S fsl4.1-tbss_4_prestats 0.2 cd FA fsl4.imglob *_FA.* cd ../stats fsl4.1-design_ttest2 design 7 6 fsl4.1-randomise -i all_FA_skeletonised.nii.gz -o tbss -m mean_FA_skeleton_mask.nii.gz -d design.mat -t design.con -n 500 --T2 -V fsl4.1-tbss_fill tbss_tfce_corrp_tstat1.nii.gz 0.95 mean_FA.nii.gz tbss_fill - could you give me any hint of formula for each step of TBSS, please? I know that it also depends on the computer you use, but I need any hint (mine is a Intel Core 2 Duo E8400 @ 3.00GHz 3.2 GB RAM that runs in ubuntu). - Is it possible to do tbss for a N=150 group of subjects? Thanks in advance, Regards, Alba Garin Simulation Area Applied Mechanics CEIT Research Centre San Sebastian - Spain