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And ambient if you were using cryo …
Edward Snell Ph.D.
Assistant Prof. Department of Structural Biology, SUNY Buffalo,
Senior Scientist, Hauptman-Woodward Medical Research Institute
700 Ellicott Street, Buffalo, NY 14203-1102
Phone: (716) 898
8631 Fax: (716) 898 8660
Skype: eddie.snell Email:
[log in to unmask]
Telepathy: 42.2 GHz
Heisenberg was probably here!
From: CCP4 bulletin board
[mailto:[log in to unmask]] On Behalf Of Poul Nissen
Sent: Thursday, January 26, 2012 10:50 AM
To: [log in to unmask]
Subject: Re: [ccp4bb] No diffraction
But first of all: try to add a synchrotron to the crystals
Poul
On 26/01/2012, at 16.48, Katherine Sippel wrote:
Might I suggest consulting the
CCP4 user community wiki on the topic:
http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Improving_crystal_quality
Good luck,
Katherine
On Thu, Jan 26, 2012 at 9:33 AM, Theresa H. Hsu <[log in to unmask]> wrote:
Dear crystallographers
I have a protein of 90 kDa forming dimers. Crystals formed with microbatch and
vapor diffusion method in 24 hours but no diffraction at home source. Dissolved
crystals was confirmed to be the protein with mass spec.
Any suggestions to improve diffraction would be welcome.
Thanking you in advance.
Theresa