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Dear all, I recently collected a dataset (~2000 frames) from a single crystal. If merge first 600 frames (sca1) or last 600 frames (sca2), Rmerge values from scalepack seem to be ok (~10%) though rejection ratios are high (~5%). But if I merge all frames together, Rmerge value goes up to ~20% and rejection is extremely high (~20%). Then, I checked sca1 and sca2 by xtriage in phenix. Surprisingly, the logfiles told me that sca1 is no twining while sca2 is very likely to be twinned. I never met this case before. So, I am wondering if it is possible from a non-twinned structure to a twinned structure just due to radiation damage. If the answer is yes, does it mean that I should not collect a large number of frames to amplify anomalous signals by using this crystal? Thanks a lot! Best, Zhiyi