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Dear all, Roche carry both glycerol and non glycerol blanking reagent, you may ask for glycerol blank kit or just send the specimen to some one has this test running at his lab if you do not have it at your lab, My experience that specimen with no visible turbidity and high trig result most likely is due to high glycerol, Please let us know your finding, Best regards Mohmed USA Sent from my iPhone On Jan 20, 2012, at 4:46 AM, "Robertson, Ken" <[log in to unmask]> wrote: > Hi all, > Off the top of my head, assuming that the Roche (?) method doesn't actually blank for existing glycerol (I do not know if it does or not but I know that many routine reagents do), I understood that "in the normal person", the glycerol would contribute about 11% to the "total TG" value (say 0.1 - 0.2 mmol/L range). This is fine in normal people but introduce (a) even relatively mild illness and (b) other possible external sources of glycerol, and I think you have a confused situation quickly as the usual balances are disrupted. Sadly, I feel that many assumptions which labs make are based upon "normal people" (whatever they are!) and may not necessarily represent the situation with the samples/patients we see in real life in most labs around the world. > > Even if there is an external source of glycerol, the question would be how long would it hang around in that state rather than get caught up in the usual TG:glycerol equilibrium. Wish I knew that. Was this person allegedly fasting for a number of hours? > > I would suggest that you will never know without a direct measurement of the glycerol content. I know that at least one reliable glycerol kit is available. > > All fun this game, isn't it? Enjoy your weekends all. > > Cheers, Ken > > > (Mr) Ken Robertson > Senior Scientist in Charge (Research) > Lipoprotein Research Lab > Core Clinical Pathology and Biochemistry > PathWest Lab Med WA > Royal Perth Hospital > Wellington Street > Perth, Western Australia 6000 > > > -----Original Message----- > From: Clinical biochemistry discussion list on behalf of John Martin > Sent: Fri 20-Jan-12 17:16 > To: [log in to unmask] > Subject: Re: Potential triglyceride interference > > > Roger is correct. The information is usually in the small print re:kit insert. > > If I remember correctly I think the endogenous glycerol conc' is ~0.1mmol/l and under normal circumstances dosen't cause many problems even if you are using the Friedewald formula to calculate LDL. > > John Martin > >> Date: Fri, 20 Jan 2012 00:01:57 +0000 >> From: [log in to unmask] >> Subject: Re: Potential triglyceride interference >> To: [log in to unmask] >> >> Just to be nitpicky: >> >> Reference to a glycerol "interference" in triglyceride assays is specious since they measure glycerol (after hydrolysis of triacylglycerides). It's similar to suggesting that poppy seeds cause an interference in opiate assays. "Interference" should refer to an analytical bias caused by something the assay was not intended to measure. >> >> Regards, >> >> Roger >> >> Roger L. Bertholf, PhD >> Professor of Pathology >> Director of Clinical Chemistry, Toxicology, >> and Point of Care Testing >> University of Florida Health Science Center/Jacksonville >> >> -----Original Message----- >> From: Clinical biochemistry discussion list [mailto:[log in to unmask]] On Behalf Of Rita Horvath >> Sent: Thursday, January 19, 2012 6:35 PM >> To: [log in to unmask] >> Subject: Re: Potential triglyceride interference >> >> Although it is only an experimental animal model but might be relevant for this discussion: >> >> Metabolism. 1979 Feb;28(2):97-9.Effect of glycerol on triglyceride metabolism in the rat. >> Abe R, MacDonald I, Maruhama Y, Goto Y. >> >> Abstract >> Lipid metabolic studies were carried out on the male Wistar rats fed on glycerol-rich diet in order to elucidate the mechanism of glycerol-induced hypertriglyceridemia. No difference was found between the glycerol fed rats and the control rats in the rate of triglyceride secretion from the liver measured by the Triton WR-1339 method as well as in the rate of incorporation of labeled glycerol into liver triglyceride. The facts that the half-life of the intravenously injected Intralipid in the blood was significantly delayed in the glycerol fed rats and that the lipoprotein lipase activity released from epididymal adipose tissue of the glycerol fed rats was markedly decreased to 19% of that of the control rats seem to account for the serum triglyceride elevation induced by the glycerol feeding. >> >> >> Regards, Rita >> Prof. Andrea Rita Horvath, MD, PhD, EurClinChem, FRCPath, FRCPA >> Past President of the European Federation of Clinical Chemistry and Laboratory Medicine >> Clinical Director, SEALS North, Department of Clinical Chemistry >> Level 4, Campus Centre, Prince of Wales Hospital >> Barker Street, Randwick, NSW 2031, Sydney, Australia >> Tel: (+612)-9382 9078 >> Fax: (+612)-9382 9099 >> Mobile No: (+61)-404 027 843 >> >> >> >> >> -----Original Message----- >> From: Clinical biochemistry discussion list [mailto:[log in to unmask]] On Behalf Of Jonathan Kay >> Sent: Friday, 20 January 2012 10:20 AM >> To: [log in to unmask] >> Subject: Re: Potential triglyceride interference >> >> Is the interference mole per mole in that assay? >> >> I don't think it could happen after ingestion: the gut and liver would metabolise it. >> >> But perhaps it could happen with topical contamination, as with the widely-observed contamination of PoCT glucose sticks by drinks containing glucose. >> >> Have you got the original specimen? >> >> Jonathan >> >> >> >> >> On 19 Jan 2012, at 22:57, David Wright wrote: >> >>> Roche Trig GPO PAP Cat No 11876023 216 >>> >>> Elderly lady, lipid profile previously normal Oct & Nov 11, presents with grossly elevated triglyceride (92.1), otherwise normal profile. >>> Serum without evidence of chylomicrons etc. Reaction trace on diluted sample normal. Indices normal (slight haemolysis 136). >>> Repeated sample 2 days later, normal (1.55) >>> >>> Diagnosis - cough >>> >>> Method (Roche Modular P) indicates glycerol may interfere. Cough meds often contain glycerol. Coincidence? Can anyone confirm such a dramatic effect before I go down to the chemist! >>> >>> David Wright >>> Biochemistry Dept >>> Antrim Laboratory >>> >> >> ------ACB discussion List Information-------- >> This is an open discussion list for the academic and clinical community working in clinical biochemistry. >> Please note, archived messages are public and can be viewed via the internet. 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Views expressed are those of the individual and they are responsible for all message content. >> ACB Web Site >> http://www.acb.org.uk >> Green Laboratories Work >> http://www.laboratorymedicine.nhs.uk >> List Archives >> http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html >> List Instructions (How to leave etc.) >> http://www.jiscmail.ac.uk/ >> >> ------ACB discussion List Information-------- >> This is an open discussion list for the academic and clinical community working in clinical biochemistry. >> Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. >> ACB Web Site >> http://www.acb.org.uk >> Green Laboratories Work >> http://www.laboratorymedicine.nhs.uk >> List Archives >> http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html >> List Instructions (How to leave etc.) >> http://www.jiscmail.ac.uk/ > > ------ACB discussion List Information-------- > This is an open discussion list for the academic and clinical community working in clinical biochemistry. > Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. > ACB Web Site > http://www.acb.org.uk > Green Laboratories Work > http://www.laboratorymedicine.nhs.uk > List Archives > http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html > List Instructions (How to leave etc.) > http://www.jiscmail.ac.uk/ > > ------ACB discussion List Information-------- > This is an open discussion list for the academic and clinical community working in clinical biochemistry. > Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. > ACB Web Site > http://www.acb.org.uk > Green Laboratories Work > http://www.laboratorymedicine.nhs.uk > List Archives > http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html > List Instructions (How to leave etc.) > http://www.jiscmail.ac.uk/ ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk Green Laboratories Work http://www.laboratorymedicine.nhs.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/