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Dialing, Without seeing your electron density maps or the protein model you have so far, it is difficult to try to explain what exactly is happening. One thought that comes to mind, however, is that there may be dual positions for these two residues. Is there any additional information you can provide? Bradley On Thu, Dec 22, 2011 at 12:12 AM, Dialing Pretty <[log in to unmask]> wrote: > Dear all, > > I have a coot blob, part of it is about 2 residues long (at the end of the > whole blob), however that part of blob is extremely close to the main-chain > of a protein fragment, and I regard that part of blob could not be explained > as 2 residues, otherwise the 2 residues would be extremely overlap with the > main chain. > > I am looking forward to getting your explanation on how to process the > 2-residue blob part. > > Cheers, > > Dialing > > -- Bradley Kearney, Graduate Student Department of Molecular and Structural Biochemistry North Carolina State University