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Dear all, Stupid question: if i do density modification (flattening and extension say from 6 to 4Å) w/o averaging on a symmetrical, say dimer, would you expect the thing stays symmetric nevertheless? (in particular if there is a strong peak in the NCS??) (seems to me it doenst necessarily...) favorite programs for NCS averiging?? DM obviously, and solomon doesnt do it. Others that do it (with out a PDB or atoms to derive it from, but rather operators) Thanks, Tommi Tommi Kajander, Ph.D., Docent Structural Biology and Biophysics Institute of Biotechnology University of Helsinki Viikinkaari 1 (P.O. Box 65) 00014 Helsinki Finland p. +358-9-19158903 [log in to unmask]