Dear Shahrzad, for calculating the Jacobian I am using the function spm_def2det from the HDW toolbox. This toolbox is provided with spm8, but it seems that the mex-files of that toolbox were not created (which is odd, because spm provides these files). Please check that your spm8 installation is correct. In order to compile the c-files, go to the spm8 folder and type "make toolbox". Regards, Christian ____________________________________________________________________________ Christian Gaser, Ph.D. Departments of Psychiatry and Neurology Friedrich-Schiller-University of Jena Jahnstrasse 3, D-07743 Jena, Germany Tel: ++49-3641-934752 Fax: ++49-3641-934755 e-mail: [log in to unmask] http://dbm.neuro.uni-jena.de On Wed, 23 Nov 2011 01:34:58 -0800, shahrzad kharabian <[log in to unmask]> wrote: >Dear SPM useres, I am very new to SPM and am planning to do a simple VBM analysis using VBM8. I have 244 T1 images (which have been converted from DICOM format using the DICOM import option of the SPM8. Using the default options of the VBM8 toolbox, I get the following error in the very first subject: Failed� 'VBM8: Estimate & Write' Undefined function or method 'spm_def2det' for input arguments of type 'single'. In file "/afs/cbs.mpg.de/software/spm/toolboxes/vbm8/0.331/cg_vbm8_write.m" (v366), function "cg_vbm8_write" at line 744. In file "/afs/cbs.mpg.de/software/spm/toolboxes/vbm8/0.331/cg_vbm8_run.m" (v364), function "run_job" at line 233. In file "/afs/cbs.mpg.de/software/spm/toolboxes/vbm8/0.331/cg_vbm8_run.m" (v364), function "cg_vbm8_run" at line 89. The following modules did not run: Failed: VBM8: Estimate & Write It seems that the error happens when it wants to write the Jacobian matrix and specifically during "estimating a more accurate jacobian determinant". I would be very thankful if someone would be able to make it for me clear what am I doing wrong and how it should be solved.� Bests, Shahrzad�