John, I was just looking into this and found the same thing. Different rp file values with reoriented images. hdr(1).private.mat -3.0000 -0.0000 0 97.5000 -0.0000 3.0000 0.0000 -96.9129 0.0000 0 4.5000 -56.4129 0 0 0 1.0000 hdr(1).private.mat0 -3.0000 0 0 97.5000 0 3.0000 0 -96.9129 0 0 4.5000 -56.4129 0 0 0 1.0000 and reoriented file: hdr2(1).private.mat -3.0000 -0.0000 0.0000 97.5000 0.0000 3.0000 0.0000 -56.9129 -0.0000 0 4.5000 -86.4129 0 0 0 1.0000 hdr2(1).private.mat0 -3.0000 0 0 97.5000 0 3.0000 0 -96.9129 0 0 4.5000 -56.4129 0 0 0 1.0000 It looks like the .mat fields are slightly different; however, the same for all volumes. The question is thus, why are the realignment parameters different if the same data is used, just different transformation matrices. Additionally, since the rp files are different, using the motion regressors as covariates would change the models as well. Are the differences due to interpolation to produce the mean image resulting in slightly different values? Best Regards, Donald McLaren ================= D.G. McLaren, Ph.D. Postdoctoral Research Fellow, GRECC, Bedford VA Research Fellow, Department of Neurology, Massachusetts General Hospital and Harvard Medical School Office: (773) 406-2464 ===================== This e-mail contains CONFIDENTIAL INFORMATION which may contain PROTECTED HEALTHCARE INFORMATION and may also be LEGALLY PRIVILEGED and which is intended only for the use of the individual or entity named above. If the reader of the e-mail is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you are in possession of confidential and privileged information. Any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited and may be unlawful. If you have received this e-mail unintentionally, please immediately notify the sender via telephone at (773) 406-2464 or email. On Fri, Nov 25, 2011 at 3:43 PM, John Ashburner <[log in to unmask]>wrote: > I don't have any ideas here. I think you really need to take a close > look at the data. You can read the fields in the headers by: > > P='blah.nii'; > Nii=nifti(P) > > If you want to see the actual values, then you can look at a hidden > field of the nifti object: > > Nii.hdr > > Computing difference images via ImCalc, and displaying them via > CheckReg can also help to give clues. > > Best regards, > -John > > On 24 November 2011 10:53, Joshua Balsters <[log in to unmask]> wrote: > > Dear SPMers > > > > I found one previous discussion on this point but not much else, so > apologies if I'm repeating what everyone already knows. > > > > I've recently starting using 4D files straight from the scanner (Philips > Acheiva) in SPM rather than splitting them into 3D files using fslsplit. > I'm finding in most cases there are subtle (+/-0.5mm) to drastic (over 2mm) > differences in my realignment parameters depending on whether I use the 4D > or 3D versions of the same datasets. These differences are only in the > translations and not the rotations. > > > > It would be great if anyone could explain why this happens. I had a > quick look for differences between the 3D and 4D files and I think the > issue is that fslplit is automatically changing my datatype from int16 to > float32, and i read in a previous discussion that it also changes the > scaling factor which is probably the bigger issue. > > > > any advice on this issue would be greatly appreciated! > > > > Best > > Josh > > >