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Dear SPMers, I guess such issue had already been addressed before, but I managed not to find the answer when searching the archive. In my study, every subject was scanned twice in two different days. The design, procedure, and all parameters were identical for both days, each having two runs of functional scanning and one T1 structural image. The only difference is that different mood was induced before scanning. So I am worrying about the preprocessing. If I want to do whole-brain analyses in MNI space, like discovering significant interaction of Mood (happy/sad) x Task (face/object), what were the correct steps for preprocessing? Should I coregister the T1 image from day1 to the T1 from day2, writing the parameter to functional images from day2, and normalise all funcional ones to EPI? Or may I simply normalise all functional ones to EPI without any coregistration? Many thanks and best, Zhenhao ----- Zhenhao SHI Culture and Social Cognitive Neuroscience Lab Department of Psychology Peking University 5 Yiheyuan Road Beijing 100871, P.R.China