All the currently used diffraction image processing programs can tell the crystal orientation from a single diffraction image. Some programs like d*TREK can calculate new orientations for the crystal if the crystal is mounted on a reasonable goniometer such as the Rigaku Kappa goniostat or the Rigaku quarter-chi goniostat. :) And the strategy program in d*TREK can use a list of previously collected and/or processed Bragg reflections to make sure that the new strategy fills in the missing parts of reciprocal space. Jim ________________________________ From: CCP4 bulletin board [[log in to unmask]] on behalf of yanwu huo [[log in to unmask]] Sent: Thursday, November 17, 2011 4:00 PM To: [log in to unmask] Subject: [ccp4bb] crystal orientation during data collection Hi, I worked on a crystal sensitive to radiation damage, So I need to merge many crystal to obtain complete dataset, Does anyone know such program that can tell crystal orientation after first frame exposure. Thank you in advance. -- Thank you very much and all the best, Yanwu Huo Postdoctoral Associate Department of Molecular Biology and Genetics Cornell University Ithaca, NY, 14853 Email:[log in to unmask]<mailto:[log in to unmask]>