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type="cite">Yanwu,
I surmise from your question that you are inquiring how to go
about collecting from many crystals optimally. Merging data ex
post facto is a totally different kettle of fish.
In my opinion, the most robust way to go about this is to use
a kappa goniometer as Jim suggested (I am most familiar with the
MK3). Since you intend to collect from many crystals, align the
first and all subsequent crystals to the same easily attainable
(or seemingly so) orientation, and then collect the sweep
suggested by your data collection strategy program of choice.
To achieve this at NE-CAT, we have a GUI-based system that
used STAC for orientation determination and BEST for strategy
generation. As Jim suggested, more options than STAC exist.
If anyone is unable to get to a kappa goniometer, they can
employ Mosflm or XDS (Xplan) to generate strategies for data
collection from a crystal taking into account previously
collected data. This is not nearly as robust a solution, but is
a workable substitute (and also automated at NE-CAT).
I know there are other ways to achieve similar results, but I
have suggested the methods I am most familiar with...
Yours,
Frank Murphy
Begin forwarded message:
Date: November
17, 2011 4:00:06 PM CST
Subject: [ccp4bb]
crystal orientation during data collection
Hi,
I worked on a crystal sensitive to radiation damage, So I
need to merge many crystal to obtain complete dataset, Does
anyone know such program that can tell crystal orientation
after first frame exposure.
Thank you in advance.
--
Thank you very much and all the best,
Yanwu Huo
Postdoctoral Associate
Department of Molecular Biology and Genetics
Cornell University
Ithaca, NY, 14853
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