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I believe you achieve completeness more quickly (fewer crystals) if you just take random orientations. At least, that's what I learnt from Dave Stuart. phx On 18/11/2011 04:20, Frank Murphy wrote: > Yanwu, > > I surmise from your question that you are inquiring how to go about > collecting from many crystals optimally. Merging data ex post facto is > a totally different kettle of fish. > > In my opinion, the most robust way to go about this is to use a kappa > goniometer as Jim suggested (I am most familiar with the MK3). Since > you intend to collect from many crystals, align the first and all > subsequent crystals to the same easily attainable (or seemingly so) > orientation, and then collect the sweep suggested by your data > collection strategy program of choice. > > To achieve this at NE-CAT, we have a GUI-based system that used STAC > for orientation determination and BEST for strategy generation. As Jim > suggested, more options than STAC exist. > > If anyone is unable to get to a kappa goniometer, they can employ > Mosflm or XDS (Xplan) to generate strategies for data collection from > a crystal taking into account previously collected data. This is not > nearly as robust a solution, but is a workable substitute (and also > automated at NE-CAT). > > I know there are other ways to achieve similar results, but I have > suggested the methods I am most familiar with... > > > Yours, > Frank Murphy > > > Begin forwarded message: > >> *From: *yanwu huo <[log in to unmask] <mailto:[log in to unmask]>> >> *Date: *November 17, 2011 4:00:06 PM CST >> *To: *[log in to unmask] <mailto:[log in to unmask]> >> *Subject: **[ccp4bb] crystal orientation during data collection* >> *Reply-To: *yanwu huo <[log in to unmask] >> <mailto:[log in to unmask]>> >> >> Hi, >> I worked on a crystal sensitive to radiation damage, So I need to >> merge many crystal to obtain complete dataset, Does anyone know such >> program that can tell crystal orientation after first frame exposure. >> Thank you in advance. >> >> >> -- >> Thank you very much and all the best, >> >> Yanwu Huo >> Postdoctoral Associate >> Department of Molecular Biology and Genetics >> Cornell University >> Ithaca, NY, 14853 >> Email:[log in to unmask] <mailto:[log in to unmask]> >> > > > >