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Ho Leung Ng wrote: > There is at least one paper describing the success of PEG precipitants > for complexes, but I can't find the reference right now. > Is this it? http://scripts.iucr.org/cgi-bin/paper?S0021889802013973 J. Appl. Cryst. (2002). 35, 674-676 [ doi:10.1107/S0021889802013973 ] Crystallization of protein-protein complexes S. Radaev and P. D. Sun Abstract: Crystallizing protein-protein complexes remains a rate-limiting step in their structure characterization. Crystallization conditions for the known protein-protein complexes have been surveyed in both the Protein Data Bank and the BMCD database. Compared with non-complexed proteins, crystallization conditions for protein-protein complexes are less diverse and heavily favor (71% versus 27%) polyethylene glycols (PEG) rather than ammonium sulfate or other high-salt crystallization conditions. The results suggest that the stability of protein complexes limits their available crystallization configuration space. Based on the survey, a set of sparse-matrix screen conditions was designed. > > Ho > > > Ho Leung Ng > University of Hawaii at Manoa > Assistant Professor, Department of Chemistry > [log in to unmask] > > -------------------------------------------------------------------- > Date: Thu, 17 Nov 2011 14:03:44 -0600 > From: Yi-Liang Liu<[log in to unmask]> > Subject: Protein-Protein Complex Screening > > Hi CCP4ers, > > I know it is not quiet related to CCP4. I am now working on a > protein-protein complex system. I am wondering which kits I should try > in a higher priority? I appreciate everyone's suggestions, and maybe > there are some papers I can read first. > > Lucas >