Hi Torsten, ad 1.: yes. Just no space inbetween "filtered_func_warp" and ".txt". ad 2.: yes, before. But I would try without (as you are motion correcting your data). Cheers, Andreas Am 17.10.11 14:12 schrieb "Torsten Ruest" unter <[log in to unmask]>: >Hi Steve, > >sorry for the confusion, I may not have been writing out things properly, >will do this now: > >1.I first use feat to pre-process the subjects data, ie. Motion >correction, B0 unwarping, smoothing and high-pass filtering. I also do >the linear/nonlinear registration to highres and then to standard space. > >2.Then I bring the individuals filtered_func data into standard space: >applywarp --ref=${FSLDIR}/data/standard/MNI152_T1_2mm --in=$filtered_func >--warp=$warp --premat=$premat out=$output > >3.I apply command-line melodic in temporal concatenation mode on standard >space data. Note I run it on all subjects (patients and controls) without >specifying any contrasts: >melodic -i filtered_func_warp .txt -o melodic -a concat --nomask --nobet >--mmthresh=0.5 --tr=3 --report -v Oall > >4.Then I run dual_regression, specifying the groups. Note I included a >3rd EV column to account for slightly different acquisition parameters >(ie there are a several subjects where the acquisition matrix was >slightly different from the majority of subjects, I attach the simple >design) >dual_regression melodic_ados/melodic_IC 1 design.mat design.con 5000 >melodic_dual_reg `cat filtered_func_warp .txt ` > >So my questions: >1. Does this procedure look OK to you? Particularly the simple design... >2. If I wanted to include the motion parameters, I'd need to use fsl_glm >before? Is this generally recommended to do? > >Thank you so much in advance for your patience. > >All the best, > >Torsten >