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Hi,
we currently use Amicon Ultra with some proteins, but with some others we also have seen precipitation problems.
In those cases we were happy with Vivacon (from Sartorius as well). They are thought for DNA but they
work also with proteins. They resemble the Centricon devices which were previously produced by Millipore.

Claudia



2011/10/6 Ho Leung Ng <[log in to unmask]>
Hello Jan,

     I've used both with soluble and membrane proteins happily. I haven't seen significant differences with protein behavior or performance in one or the other, but of course, your favorite protein may differ. I've found that buffer condition is a more important variable than membrane type.


Ho

Ho Leung Ng
University of Hawaii at Manoa
Assistant Professor, Department of Chemistry
[log in to unmask]



--
Claudia Binda
University of Pavia
Dept. Genetics and Microbiology
via Ferrata 1, 27100 Pavia - Italy
Phone: +39-0382-985535
Fax: +39-0382-528496
E-mail: [log in to unmask]
Web: http://www.unipv.it/biocry