One more thing you could try: high pressure cryo-cooling. Se any of a
number of paperas by Chae Un Kim; e.g.
http://www.ncbi.nlm.nih.gov/pubmed/17452791
Acta Crystallogr D Biol Crystallogr.
<http://www.ncbi.nlm.nih.gov/pubmed/17452791#> 2007 May;63(Pt 5):653-9.
Epub 2007 Apr 21.
On 10/26/11 12:46, Leonard Thomas wrote:
> Hi All,
>
> I have run into a very sensitive crystals system when it comes to cryo
> protecting them. I have run through the usual suspects and trays are
> going to be setup with a cryo protectant as part of crystallization
> cocktail. The one problem that seems to be occurring is that the
> crystals crack as soon as they are transfered out of the original
> drop. I am running out of ideas and really would love some new ones.
>
> Thanks in advance.
>
> Len
>
> Leonard Thomas Ph.D.
> Macromolecular Crystallography Laboratory Manager
> University of Oklahoma
> Department of Chemistry and Biochemistry
> Stephenson Life Sciences Research Center
> 101 Stephenson Parkway
> Norman, OK 73019-5251
>
> [log in to unmask]
> http://barlywine.chem.ou.edu
> Office: (405)325-1126
> Lab: (405)325-7571
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All Things Serve the Beam
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David J. Schuller
modern man in a post-modern world
MacCHESS, Cornell University
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