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Hi - MELODIC will take care of this for you. The number of components is up to you - look at the GUI and the manual - you can specify the number yourself, or ask MELODIC to estimate it for you. Cheers. On 6 Sep 2011, at 05:34, Debby Klooster wrote: > Dear FSL users, > > I am using FSL MELODIC to perform ICA on resting state fMRI datasets. I also use another program to do a general (GLM) analysis. I would like to use the preprocessed data of the other program as input in FSL, which works easily because it is also NifTi format. However, from the literature I know that it is necessary to center the data and to perform a whitening step. I would like to know in which step FSL is doing this? Is that automatically implemented in MELODIC? Otherwise I might have to implement those steps by hand. > > Besides that, I was wondering how many components you use. Do you make MELODIC calculate the optimum number of components or do you pre-define the number? I am doubting what to do since I get a lot of components when I make MELODIC define the number. On the other hand, I don't want to use a number that is too low since my components will not all be split by then. > > Thank you very much! > Debby Klooster > --------------------------------------------------------------------------- Stephen M. Smith, Professor of Biomedical Engineering Associate Director, Oxford University FMRIB Centre FMRIB, JR Hospital, Headington, Oxford OX3 9DU, UK +44 (0) 1865 222726 (fax 222717) [log in to unmask] http://www.fmrib.ox.ac.uk/~steve ---------------------------------------------------------------------------