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>Is it possible to precipitate proteins (TCA, acetone) from a sample >that has already been stored in protein loading dye? The protein is >too dilute in my current sample and I basically want to load all of >the sample (100uL) in a single well in the gel. Unfortunately, I >already added protein dye with SDS and all. Methanol/chloroform precipitation is both more effiecient than TCA or acetone and it can do exactly what you want it to do. Wessel, D., and Fugge, U.I. A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids. Analytical Biochemistry, 1984, 138:141-143 Or google it - there are many online protocols. - Dima