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Hi Gareth, We performed a small collaborative study with our NOVA POC meters for ketones and the main issue was with sample viscosity in post mortem samples. We did try to overcome this with protease treatment but to no avail. As Dave said the preferred method is GCMS. Also the methods are not standardised so you will see between method variations, so the acceptability should be assessed in relation to the clinical context. Regards, Kayleigh Kayleigh Davis Trainee Clinical Scientist 4th Floor, North Wing St. Thomas' Hospital Westminster Bridge Road London SE1 7EH United Kingdom ________________________________ From: Clinical biochemistry discussion list [mailto:[log in to unmask]] On Behalf Of GARETH DAVIES (BCUHB - Pathology) Sent: 29 September 2011 09:53 To: [log in to unmask] Subject: Hydroxybutyrate in PM samples Dear All, I've just had a query from one of our Histopathologists regarding the measurement of hydroxybutyrate in post mortem blood to suggest alcoholic ketoacidosis. He has some papers which recommend this but none of them give details on what analytical methods are used. We use the Abbott Optium Xceed meters for POCT ketone analysis in whole blood (in living patients), has anyone used this for PM samples? Many thanks. Gareth Gareth Davies Biocemegydd Clinigol/Clinical Biochemist Bwrdd Iechyd Prifysgol Betsi Cadwaladr University Health Board Ysbyty Maelor Wrecsam/Wrexham Maelor Hospital Wrecsam/Wrexham LL13 7AS 01978 725345 P Please consider the environment before printing this email or its attachment(s) ________________________________ Cymraeg Rhybudd Ebost (2010) - Bwrdd Iechyd Prifysgol Betsi Cadwaladr Fe'ch cynghorir i ddarllen rhybydd ebost Bwrdd Iechyd Prifysgol Betsi Cadwaladr (a'i argraffu er mwyn cyfeirio ato yn y dyfodol). Gellir dod o hyd iddo yn y lleoliad canlynol http://www.wales.nhs.uk/sitesplus/861/tudalen/47230 <http://www.wales.nhs.uk/sitesplus/861/tudalen/47230> Bwrdd Iechyd Prifysgol Betsi Cadwaladr yw enw gweithredol Bwrdd Iechyd Lleol Prifysgol Betsi Cadwaladr. English Betsi Cadwaladr University Health Board - Email Notice (2010) You are advised to read (and print for future reference) the Betsi Cadwaladr University Health Board e-mail notice which can be found at this location http://www.wales.nhs.uk/sitesplus/861/page/47229 <http://www.wales.nhs.uk/sitesplus/861/page/47229> Betsi Cadwaladr University Health Board is the operational name of Betsi Cadwaladr University Local Health Board ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk Green Laboratories Work http://www.laboratorymedicine.nhs.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/ ************************************************************************************* The information contained in this message and or attachments is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Unless otherwise specified, the opinions expressed herein do not necessarily represent those of Guy's and St Thomas' NHS Foundation Trust or any of its subsidiaries. The information contained in this e-mail may be subject to public disclosure under the Freedom of Information Act 2000. Unless the information is legally exempt from disclosure, the confidentiality of this e-mail and any replies cannot be guaranteed. Any review, retransmission,dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and delete the material from any system and destroy any copies. We make every effort to keep our network free from viruses. However, it is your responsibility to ensure that this e-mail and any attachments are free of viruses as we can take no responsibility for any computer virus which might be transferred by way of this e-mail. ************************************************************************************* ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk Green Laboratories Work http://www.laboratorymedicine.nhs.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/