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Dear Bernardo, I'm afraid that FIRST cannot segment the corpus callosum at present. However, you can segment the white matter with FAST and then extract the hemispheric plane with a FLIRT transformation to standard space (or FNIRT if you think there is benefit in having it non-linear). You would still need to extract the appropriate part manually (eliminating other white matter structures present in this plane), but it would be more efficient than a fully manual solution. All the best, Mark On 16 Aug 2011, at 13:46, Bernardo Jose Alves Ferreira Martins wrote: > Hi, > I am not sure which strategy would work better for evaluating corpus callosum thickness/area. We did it by FLIRTing 6-parameter, than measuring manually. > I was thinking if it could be done by FIRST. Is it possible? > > Cheers, > Bernardo >