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Hi all

What's a way to determining a B-factor to set for all residues of a model at low resolution (4A)? How valid is the wilson B at this resolution? (better than nothing?)

I'm in the process of fitting whole domains into some rasty experimental density maps and was thinking of doing a rigid body or very restrained  ( secondary structure | reference structure | etc ) refinement of the coordinates for helping the fit. 

Thanks!

F



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Francis E. Reyes M.Sc.
215 UCB
University of Colorado at Boulder