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Dear all, I recently obtained some spherulites while trying to crystallize my protein. The spherulites are manually reproducible, but changing pH, protein concentration, and salt concentration does not result in crystal formation. Microseeding with crushed spherulites isn't a solution either as it only yields new spherulites. Next stepp is the use of an optimization kit but I have a limited amount of material, and I start doubting that these are protein spherulites, as the spherulites are not particularly soft. The condition contains 15% PEG 3350 and 200 mM NaCl. Does anyone know if PEG 3350 forms easily spherulites around that concentration? Thanks,