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Ok, so I think the mwxi.nii should be it. best, carsten ________________________________ Von: Pablo Ripollés Vidal [[log in to unmask]] Gesendet: Dienstag, 12. Juli 2011 19:40 An: Finke, Carsten Cc: [log in to unmask] Betreff: Re: [SPM] Segmentation and Normalization Hi Carsten, you are wellcome. I have never used that option, and I do not know how it works sorry. The mask needs to have a 0 for every voxel of the lesioned area and a 1 for every voxel outside the lesioned area. What I usually do is save the voi drowing as a nii file which gives you an image with 1 for voxels inside the lesion and 0 for voxels outside it. Then I use ImageCalc to do a 1 - i1. Maybe there is a more direct and easy way to do this. Best Regards, 2011/7/12 Carsten Finke <[log in to unmask]<mailto:[log in to unmask]>> Hi Pablo, I changed the bounding box and it works - many thanks for your help! And you're right, of course, it's the Crinion-paper. And thanks for the other reference (Andersen et al 2010). As I am drawing lesion masks anyway (in MRIcron), I will give it a try. Do you know whether I just can use the "Create SPM5 masks" option in MRIcron for masking the lesion in SPM8? It creates two files (mwxi.nii and lwxi.nii), I guess I have to use mwxi.nii? carsten