Thank you! I got that now! I can't believe I forgot that *.nii was 4D image.
 
Thanks again!
 
2011-07-08

李琳玲

Linling Li
Research Centre for Neural Engineering
--------------------------------------
Phone: +86-755-86392232
Email:  [log in to unmask]
Shenzhen Institutes of Advanced Technology,
Chinese Academy of Sciences.

发件人: Christian Lambert
发送时间: 2011-07-08  15:00:09
收件人: SPM
抄送:
主题: Re: [SPM] Qestion about making whole brain T1 template using DARTEL
All your images are already in the original Template file (the one you used rC1, rC2 and rC3 to make) so you don't need to re-run each separately. See the attached image, it should clarify what John said.

- Chris

2011/7/8 Linling Li <[log in to unmask]>
Dear John,
 
Thanks for your reply!
 
Maybe I haven't describe my question in the right way because of my poor English writing and the limited knowledge of image processing algorithms. I have posted a question regarding to making tissue probability maps to this mailing list long time ago, and you gave me some explanation about this question nicely. Since I'm dealing with the data of elder subjects, I still want to try to apply a customized template to see the difference compared to appling the stanndard template.
 
So, I followed the tutorial you wrote. After using the New Segment tool of SPM8, I got C1*.nii, C2*.nii, C3*.nii  and rC1*.nii, rC2*.nii, rC3*.nii  for GM, WM and CSF. Then I want to use 'Dartel: Creat Templates' to create the template of GM, WM, and CSF which can be used as the tissue probability maps in the 'Segment' step of SPM8. Besides, I want to create the whole brain T1 template on which the group statistical results could be overlaid.  However, I found that when I used three groups of images in the 'Dartel: Creat Templates' step (three channels of images: rC1*.nii, rC2*.nii, rC3*.nii), I only got a template of GM. Then I tried to only use one channle of images (rC2*.nii), I got a template of WM.
 
Therefore, could I just run the 'Dartel: Creat Template' three times with only one channel of images of rC1*.nii, rC2*.nii or rC3*.nii in one time in order to get the templates of GM, WM and CSF which can be used as the tissue probability maps? But I'm confused with that even I used three channels of images (GM and WM and CSF), the only template I gor only contain the gray matter? So I don't know if I can create the customized wholebrain T1 template using Dartel.
 
By the way, I also want to ask you about another simple question. I want to try to do the 1st level analysis in the native space of each subject. After the realignment of functional data, I use Coregister:Estimate to register T1 anatomical image to mean functional image derived from the realignment step. Then I smoothed the unnormalized functional data and doid the 1st-level analysis. After that, I can overlay the spmT image on the subject's own T1 image. Is this right?
 
Thank you very much!
 
2011-07-08

李琳玲

Linling Li
Research Centre for Neural Engineering
--------------------------------------
Phone: +86-755-86392232
Email:  [log in to unmask]
Shenzhen Institutes of Advanced Technology,
Chinese Academy of Sciences.

发件人: John Ashburner
发送时间: 2011-07-07  18:45:53
收件人: SPM
抄送:
主题: Re: [SPM] Qestion about making whole brain T1 template using DARTEL
Use check reg to display the images, and when you select the file,
change the 1 in the user interface to 1:4 instead.  This allows more
than just the first volume to be selected.
Best regards,
-John
2011/7/7 Linling Li <[log in to unmask]>:
> Dear all,
>
> Is it possible to make a whole brain T1 template using DARTEL? I tried
> according to the tutorial wrote by
> John Ashburner(VBM Tutorial,March 15, 2010). After DARTEL(Create
> Template)(the input were rC1*.nii, rC2*.nii, rC3*.nii  in  three channels),
> the template I got only included gray matter.
>
> Thanks a lot in advance!
>
>
> 2011-07-07
> ________________________________
> 李琳玲
>
> Linling Li
> Research Centre for Neural Engineering
> --------------------------------------
> Phone: +86-755-86392232
> Email:  [log in to unmask]
> Shenzhen Institutes of Advanced Technology,
> Chinese Academy of Sciences.