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Dear all, I think spatial transformation into a common coordinate space (MNI) is not proper anymore when the subjects are elderly people and their brain is very different from the standard template applied in SPM8. So, I want to carry out first level statistical analyses in native space. Then I want to do ROI analysis based on individual T-maps derived from the 1st-level analysis. So, the question is how to define the anatomical regions of interests (ROIs) on each subject's T1 anatomical image? Hope someone who had done similar data analysis experience could give me some advice. I know a toolbox called "AutoSeg", but it is based on C++ and runs under the GUI of FLTK. Is there any auto segmentation, pacellation and labelling toolbox that works under MATLAB and SPM? Thanks a lot! 2011-07-06 ÀîÁÕÁá Linling Li Research Centre for Neural Engineering -------------------------------------- Phone: +86-755-86392232 Email: [log in to unmask] Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences.