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http://www.globalphasing.com/sharp/ or you could extend your phases using DM if you have more than one molecule in the asu. I'm sure there is also a way to convince solve/resolve to do what you want. If you see enough in your map, just build a backbone model and use that to generate a crude mask for DM (in case you can apply NCS averaging). Jürgen On Jul 12, 2011, at 9:08 PM, Jiamu Du wrote: Dear All, I am now working on a low resolution phase determination (around 3.3 A with Se anomalous signal around 3.8 A). I can find the Se site and get the phase, but the density map is not so good. Some part of the protein (about 1/3) has a homologue model which is also can be found using Phaser. The homologue region has a good map while other region only show a poor map. I think the combination of experimental phase and MR phase might improve the map. Is there anybody can help find which program can work on this? Thanks. -- Jiamu Du, Ph.D. Postdoctoral Research Fellow Laboratory of Structural Biology Memorial Sloan-Kettering Cancer Center RRL 269, 430 E 67th Street New York, NY, 10021 E-mail: [log in to unmask]<mailto:[log in to unmask]> Tel: (217) - 417 - 9897 ...................... Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/