OK, same space group, but you didn't indicate what the unit
cells were. They are different, right?
Hello and good day to everyone! :)
I have some general questions on
crystallography work. I hope you don't mind giving me some ideas.
I
have solved my lipase protein both ground-grown crystals and space-grown
crystals with good resolutions (1.4A and 2.2A). They are the same protein from
the same source, same purification methods, and produced crystals from the same
crystallization conditions (except the gravity part).
From the
data, it shows that both of them belong to the same space group P212121. But
they have different number of molecule per asymmetric unit. Ground crystal= 1
molecule/ASU, Space crystal= 2 molecules/ASU. At the moment i have problem
explaining this issue. Is it normal to have such results? Same protein with
different number of molecule/ASU?
I've been trying to get some
references on this matter but so far i don't really get anything that can
directly explain it. Furthermore, do i need to relate this with the gravity
effect?
I hope you don't mind sharing some experiences on crystallography
especially regarding this matter.
Thank you very much
--
FAIROLNIZA
"The advantage of the emotions is that
they lead us astray, and the advantage of science is that it is not
emotional"
-Oscar Wilde, The Picture of Dorian Gray, 1891