'NMR people' do use thermophilic proteins but I have never heard of anyone boiling samples :) By increasing temperature I meant going to around 37-40 deg C. On 7/1/11 12:26 PM, "Jacob Keller" <[log in to unmask]> wrote: >...they are stable enough that one can raise the temperature to increase the correlation time. I wonder whether NMR people have tried using hyperthermophile proteins to be able to essentially boil the sample to decrease the rotational correlation time constant? There is even an organism I read about in a Science brevia paper whose peak doubling time is at autoclave temperatures... JPK