I am trying to refine a protein-DNA structure, and I am having
difficulties refining the DNA using COOT. I used the ccp4i Phaser
program to molecular replace the protein and DNA simultaneously (there
are solved crystal structures of the free protein and a similar DNA
bound to another protein), but I am only able to manually refine the
protein in COOT. If I try to refine the DNA, a window pops up with the
following statement: No Restraints Found! Non-existent or minimal
description of restrained residues. How do I create a non-minimal mmCIF
dictionary for just the DNA? I am relatively new to crystallography
and would appreciate any guidance.
Brittney