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On 07/04/2011 04:24 PM, ruheng wrote: > > Dear all, > > Recently we are working on an archaebacteria protein which was expressed and purified from E.coli by conventional procedures. After we solved the structure, we found that there is an extra density in one of the argninine as shown in the attached picture. It seems that the density is larger than a methyl group and the atoms in the density are not on one plane. So we are curious about whether the density is a kind of posttranslational modification of arginine residues in the protein, if it is, what kind of modification could it be? And most important, what is the biological significance of this kind modification? Any suggestions and dicussions are appreciated! > > Best, > Heng > We saw something like this and modelled it as glycerol. But I dont know how one knows if one is correct!! Eleanor