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Dear Blake, Are you using the -2D option with the -applyxfm and -init? It is necessary in order for it to appropriately interpolate correctly. However, it does assume that the image is in the xy-plane. If this is not the case then it will require some special options. All the best, Mark On 30 Jun 2011, at 21:23, Blake Dewey wrote: > Experts, > > I have a transform matrix from a 2D 6 dof registration and I would like to apply it to another 2D image. Is this possible? I tried -init mat.mat -applyxfm and didn't work (the entire image was the same value). I also tried the GUI with the same result. > > > Thanks, > Blake Dewey > Vanderbilt University Institute of Imaging Science >