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Dear all, Could any one help me regarding my serious problem actually i have collected data at 3.0 and cut off 3.1 where the data statics showed the good values for the further processing. According to the methew coefficient there would be two molecule in the asymmetric unit but after running the molrep its provide only one monomer instead of two, for this reason R values is very high 50%. Actually homologous model having P6322 space group where as my one is F4132. I had tried to run phaser as well phenix but both were failed to process further. i really do not know how can i get the second chain in my structure. Please if you have some ideas i will appreciate and would be many thankful to you. Hope to hearing you soon Best Regards AFSHAN