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On Wednesday, June 01, 2011 04:45:36 pm Pavel Afonine wrote: > Hi Hailiang, > > to get feeling about how maps may look like at different resolutions do the > following learning exercise: download a structure from PDB and compute Fcalc > maps at different resolutions: > > phenix.fmodel model.pdb high_res=1 > phenix.fmodel model.pdb high_res=2 > phenix.fmodel model.pdb high_res=3 > phenix.fmodel model.pdb high_res=4 > phenix.fmodel model.pdb high_res=5 > ... > phenix.fmodel model.pdb high_res=10 > phenix.fmodel model.pdb high_res=20 > > then load them in Coot and you will get your answer. True. Although really you need not go through phenix repeatedly for this purpose. Coot itself lets you choose the resolution limits on input. So you only need one map or *.mtz file, which you can read in at whatever resolution you like. Ethan > > Pavel. > > > On Wed, Jun 1, 2011 at 4:35 PM, Hailiang Zhang <[log in to unmask]> wrote: > > > Hi there, > > > > I have a preliminary question. For very low resolution data, say 10A or > > even lower, is the density map supposed to be more like a big envelop > > covering the whole molecule, or more like a collection of isolated small > > envelops covering small motifs (eg helix as cylinder envelop)? I got the > > later one but need to make sure and persuade others I didn't do things > > wrong. > > > > Any references will also be appreciated! > > > > Thanks! > > > > Hailiang > > > -- Ethan A Merritt Biomolecular Structure Center, K-428 Health Sciences Bldg University of Washington, Seattle 98195-7742