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Better yet, RbBr? and pH MES, MOPS, and HEPES with RbOH? I haven't checked the price. Jacob Keller wrote: > Is there any reason why crystallographers have not routinely > substituted NaBr for NaCl in protein crystallization stocks, or even > pH'd their TRIS with HBr, if there is no NaCl? Wouldn't it make a lot > of sense, since there would always be a possibility for a Br- > derivative, and the price difference is pretty small? Also, a data set > at the Br- peak would always be able to distinguish "heavy H2O" from > weak halide sites once the structure was solved, even if the sites > were not good enough for phase determination themselves. > > JPK > > On Wed, Jun 15, 2011 at 7:35 AM, Stephen Graham<[log in to unmask]> wrote: >> You might also want to try: >> >> http://www.ncbi.nlm.nih.gov/pubmed?term=12499536 >> >> Cheers, >> >> Stephen >> >> On 15 June 2011 02:09, Robbie Joosten<[log in to unmask]> wrote: >>> Hi Wolfram, >>> >>> This was an early study on the subject: >>> http://www.ncbi.nlm.nih.gov/pubmed/8594192 >>> The software is still accessible via the STAN server. >>> >>> Cheers, >>> Robbie >>> >>>> Date: Tue, 14 Jun 2011 17:51:21 -0400 >>>> From: [log in to unmask] >>>> Subject: [ccp4bb] non-waters among structured solvent atoms >>>> To: [log in to unmask] >>>> >>>> Dear colleagues, >>>> following a discussion in our lab, I have volunteered to dig out >>>> articles from the literature about erroneous assignments of non-water >>>> entities such as metal ions, halides in protein models. For example I >>>> have the faint recollection that data mining of the PDB for suspect >>>> "water" assemblies matching the geometry of coordinated cations has >>>> previously been described. But none of my google searches has turned >>>> up the references I was looking for. Could someone point me in the >>>> right direction, please? >>>> Many thanks, >>>> Wolfram Tempel >>> >> >> >> >> -- >> Dr Stephen Graham >> 1851 Research Fellow >> Cambridge Institute for Medical Research >> Wellcome Trust/MRC Building >> Addenbrooke's Hospital, Hills Road >> Cambridge, CB2 0XY, UK >> Phone: +44 1223 762 638 >> > > > > -- > ******************************************* > Jacob Pearson Keller > Northwestern University > Medical Scientist Training Program > cel: 773.608.9185 > email: [log in to unmask] > ******************************************* >