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On Wed, Jun 1, 2011 at 4:45 PM, Pavel Afonine <[log in to unmask]> wrote: > to get feeling about how maps may look like at different resolutions do the > following learning exercise: download a structure from PDB and compute Fcalc > maps at different resolutions: > > phenix.fmodel model.pdb high_res=1 > phenix.fmodel model.pdb high_res=2 > phenix.fmodel model.pdb high_res=3 > phenix.fmodel model.pdb high_res=4 > phenix.fmodel model.pdb high_res=5 > ... > phenix.fmodel model.pdb high_res=10 > phenix.fmodel model.pdb high_res=20 > > then load them in Coot and you will get your answer. > But fake data will always look much better than real data - to start with, the B-factors will be all wrong. There are actually a handful of PDB entries that are between 8.0 and 12.0 and have experimental data deposited: 1H1K, 1PNS, 1PNX, 1VCR,1ZBB, 2QIJ, 2QZV, 3LVH, 3PCQ and 1VOQ, 1VOR, 1VOS, 1VOU, 1VOV, 1VOW, 1VOX, 1VOY, 1VOZ, 1VP0, which are all the same structure split over multiple files. I'd suggest downloading a few of these and looking at the maps. -Nat