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Dear all, We are developing a monitoring service for an anti-viral drug (ribavirin) which appears to be relatively stable in plasma but rather unstable in whole blood - particularly at room temperature - where levels decrease over the first few hours (? exchange with nucleotides in cells) followed by a large increase (leaking out of red cells, where levels are >60 times that of plasma). Stability at 4C is better (so we are proposing placing samples onto ice / straight into the fridge and separation within an hour or so). We have gone for EDTA samples as we use them for the immunosuppressants and could use the same sample for HCV RNA levels / genotyping. However we might not be using the best anticoagulant for the job. Is there any information around as to the effects of the different anticoagulants on red cell stability / membrane transporters? - we don't want to embark on a study which requires drawing a fluoride oxalate, a lithium heparin, a sodium citrate... Thanks Nigel Nigel Brown PhD FIBMS MRSC Principal Biochemist IDM Service, Institute of Liver Studies King's College Hospital, London. SE5 9RS UK Phone +44 20 3299 7639 (office), 3299 3147 (lab) Fax +44 20 3299 3641 King's College Hospital is part of King's Health Partners Academic Health Sciences Centre (AHSC), a pioneering collaboration between King's College London, and Guy's and St Thomas', King's College Hospital and South London and Maudsley NHS Foundation Trusts. For more information, visit www.kingshealthpartners.org<http://www.kingshealthpartners.org/> ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and GSi recipients NHSmail provides an email address for your career in the NHS and can be accessed anywhere For more information and to find out how you can switch, visit www.connectingforhealth.nhs.uk/nhsmail ******************************************************************************************************************** ------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry. Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content. ACB Web Site http://www.acb.org.uk Green Laboratories Work http://www.laboratorymedicine.nhs.uk List Archives http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html List Instructions (How to leave etc.) http://www.jiscmail.ac.uk/