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Hello, Tim suggests that you should click the "Pick new peaks" and "Pick non-maxima peaks" check boxes. Now what that does is that it will try and find a local extremum within the specified tolerances and if it doesn't find one it will put down a peak at the reference position. So if you want all your peaks to be at the reference position then you should make the tolerances small enough that it will then not find any extrema. The tolerances are set in the table below those two check boxes, you might need to resize the dialog to see it. Wayne On Thu, 26 May 2011, S.P. Skinner wrote: > Hi All > > I am using the follow intensity changes function to calculate R1 and R2 rates > for my protein, but there appears to be an issue wherein the peak position > changes from point to point. I have picked and assigned the first plane, but > not the others. Is there a way to force the peak picking to stay in exactly > the same place or would I have to manually assign all planes of the pseudo 3D > spectra? If the latter is true, is there a quick way to do this? > > Regards > > Simon > > -- > Simon P Skinner > Protein Chemistry Group > Leiden Institute of Chemistry, Universiteit Leiden > Phone: +31 71 527 6089 / Fax: +31 71 527 4349 > E-mail : [log in to unmask] >