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Dear All, Sorry for the off topic question. I am purifying one protein which is showing increased molecular weight +5kDa (more than adding up the Hexa His and cloning artifact) in normal 12% SDS PAGE. The DNA sequence is as it is. The gel runs without blurring the lanes and without any difficulties. My protein may contains hydrophobic patches, does it may cause retard migration. Please help me suggesting the possible reason of increased molecular weight. Thank you for your kind suggestions. Sincerely, Debajyoti