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Hello Daniel, no reason to worry, just take a look a the release note accompanying the latest update of SPM8 (4290): http://www.fil.ion.ucl.ac.uk/spm/software/spm8/SPM8_Release_Notes_r4290.pdf In fact, only the subject factor has been shifted/moved to the right-hand side of the design matrix (apart from fixing a bug). Moreover, once the design is estimated (e.g.) using release 4010 there is no need to reestimate the model for displaying results of the very same model/design using release 4290 (and vice versa), at least on my machine. Just select the model (using release 4290) that is already estimated (by release 4010) and choose the contrast of interest. Could the differing results between both releases be due to the fact that you did not adapt the contrasts to the new order of factors (subject, condition, group) in the design matrix of the new release (take a close look at the design matrix)? Hope I could help and best regards Raphael Am 20.04.2011 12:07, schrieb Daniel Ferreira: > Dear SPM experts, > > I would very appreciate if you can help me. > > Is something different in the spm8_4290 version regarding the design matrix/estimation as comparing with the spm8_4010 version? > > The thing is that I have tried to estimate the same identical flexible_factorial.mat in both versions (spm_4010 and spm_4290). I do get different design matrices (mirrored ones) and I do not get why and of course the results are completeley different. > > Can I avoid to create a new .mat for the 4290 version? I mean, can I use the 4010's one in 4290 version? > > Please find attached the design matrices. One has 6 conditions, the other one has 66. > > > Thank you very much in advance, > > Daniel Ferreira >