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Hi all, could you use its position in real space as a target for (to make it easy, rigid body) refinement? Real space meaning the electron density around the scatterer in an anomalous LLG map. Some other restraints: Say it's a metal cofactor and you know that it needs to be in a specific position with respect to your protein. F --------------------------------------------- Francis E. Reyes M.Sc. 215 UCB University of Colorado at Boulder