Kushol,

Thank you for the reply. Might I ask what kind of HPLC system you have? Our HPLC is old and has stainless steel pumps, and to our knowledge this is not compatible with the Dionex columns (due to metal leaching). Dionex has recommended either a titanium or peek system. As our HPLC is still running well, we were hoping to find a column that can tolerate the stainless steel.

Jamie


On 4/28/11 9:11 AM, "Kushol Gupta" <[log in to unmask]">[log in to unmask]> wrote:


Hi Jamie –
 
We’ve had good success with these columns from Dionex (the DNAPac PA-100 and the DNAPac PA200 (Analytical 4x250mm and Semi-Prep 9x250mm,)).  We use them precisely for the application you describe.
 
http://www.dionex.com/en-us/products/columns/bio/nucleic-acid/dnapac-pa100/lp-73370.html
http://www.dionex.com/en-us/products/columns/bio/nucleic-acid/dnapac-pa200/lp-73371.html
cheers,
 
Kushol
 

Kushol Gupta, Ph.D.
Research Associate
Van Duyne Laboratory - HHMI/Univ. of Pennsylvania School of Medicine
[log in to unmask]">[log in to unmask] <BLOCKED::mailto:[log in to unmask]>
215-573-7260 / 267-259-0082

From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Wallen, Jamie
Sent: Thursday, April 28, 2011 9:32 AM
To: [log in to unmask]">[log in to unmask]
Subject: [ccp4bb] DNA oligonucleotide purification
All,

Our laboratory is looking for a new column to purify short (40mers or less) DNA oligonucleotides that will be used for crystallization setups. I wanted to ask if anyone has suggestions of a column that will give excellent separation of N and N-1 products. We have a Shimadzu HPLC system. We are considering Dionex columns as well as Oligonucleotide Separation C18 columns from Waters, but if there are others that are better we would like to know. Any suggestions will be greatly appreciated. Thank you!

Jamie Wallen