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Hi, we recently published a method that could be just what you need, and easy to implement. It detects covalent modifications of cysteines, and mercury, gold and some platinums target cysteines... have a look at this paper. feel free to contact me if you want more information. vincent Fluorescence Detection of Heavy Atom Labeling (FD-HAL): A rapid method for identifying covalently modified cysteine residues by phasing atoms. Chaptal V, Ujwal R, Nie Y, Watanabe A, Kwon S, Abramson J. J Struct Biol. 2010 Feb 10. Le 3/25/11 8:39 AM, Careina Edgooms a écrit : > Dear ccp4 users > > I would like to know, is there a way to check that heavy metal has > bound to crystals before I take it to synchrotron which is far away? > > thanks > Careina > -- Vincent Chaptal, PhD Institut de Biologie et Chimie des Protéines Drug-resistance modulation and mechanism Laboratory 7 passage du Vercors 69007 LYON FRANCE +33 4 37 65 29 01 http://www.ibcp.fr