Hi,

we recently published a method that could be just what you need, and easy to implement.
It detects covalent modifications of cysteines, and mercury, gold and some platinums target cysteines...

have a look at this paper.
feel free to contact me if you want more information.
vincent

Fluorescence Detection of Heavy Atom Labeling (FD-HAL): A rapid method for identifying covalently modified cysteine residues by phasing atoms.

Chaptal V, Ujwal R, Nie Y, Watanabe A, Kwon S, Abramson J.

J Struct Biol. 2010 Feb 10.

Le 3/25/11 8:39 AM, Careina Edgooms a écrit :

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Dear ccp4 users

I would like to know, is there a way to check that heavy metal has bound to crystals before I take it to synchrotron which is far away?

thanks
Careina

Vincent Chaptal, PhD

Institut de Biologie et Chimie des Protéines

Drug-resistance modulation and mechanism Laboratory

7 passage du Vercors

69007 LYON

FRANCE

+33 4 37 65 29 01

http://www.ibcp.fr