Dear Henry, This one is actually correct, I think. pyroglutamic acid has an internal amide, and the only reactive group is the acid group. When that is involved in a link (as it is, for 'start') there is nothing left to protonate or deprotonate. Yours, Rasmus --------------------------------------------------------------------------- Dr. Rasmus H. Fogh Email: [log in to unmask] Dept. of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK. FAX (01223)766002 On Fri, 18 Mar 2011, Henry Hocking wrote: > Dear developers, > > I noticed that pyroglutamic acid (Ccp Code Pca) exists only in neutral > form when it's 'Polymer linking' is 'start'. Shouldn't there be an option > for 'prot:H1' at this position? > Kind regards, > > Henry > > > > -------------------------------------------------------- > Henry Hocking, PhD > > Utrecht University > Bijvoet Center for Biomolecular Research > Padualaan 8 > 3584 CH Utrecht, The Netherlands > > Phone: +31-30-2532875 > Fax: +31-30-253 7623 > E-mail: [log in to unmask] > > > > > > >